--- title: Delmic 博客 | cryo-electron tomography description: cryo-electron tomography | Delmic 博客 --- [![](https://blog.delmic.com/hs-fs/hubfs/Social%20Media/CRYO_Ice%20Contamination_SocialMedia_Labeled.png?width=620&name=CRYO_Ice%20Contamination_SocialMedia_Labeled.png) ](https://blog.delmic.com/cn-blog/preventing-ice-contamination-in-the-cryo-et-workflow) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ## [冷冻电镜 | cryo-ET工作流程中的冰污染问题及其解决方法](https://blog.delmic.com/cn-blog/preventing-ice-contamination-in-the-cryo-et-workflow) 冷冻电子断层扫描（cryo-ET）是一种非常强大的技术，可以在接近原生状态下对细胞结构进行高分辨率的研究。由于厚于600纳米的生物样品不对电子透明，因此需要减薄才能用于冷冻电子断层扫描。为了使样品变薄，使用带有聚焦离子束和冷冻载物台的扫描电子显微镜(cryo-FIB/SEM)已成为黄金标准。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/METEOR_MillingWorkflow_Blogpost.png?width=380&name=METEOR_MillingWorkflow_Blogpost.png) ](https://blog.delmic.com/cn-blog/focused-ion-beam-sample-preparation-for-cryo-electron-tomography) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [用于冷冻电子断层扫描的聚焦离子束（FIB）样品制备](https://blog.delmic.com/cn-blog/focused-ion-beam-sample-preparation-for-cryo-electron-tomography) [![](https://blog.delmic.com/hs-fs/hubfs/DMVPores_SARS-CoV-2.png?width=380&name=DMVPores_SARS-CoV-2.png) ](https://blog.delmic.com/cn-blog/cryo-et-imaging-of-sars-cov-2-replication-organelles) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [冷冻电子断层扫描的应用：成像新冠病毒复制细胞器](https://blog.delmic.com/cn-blog/cryo-et-imaging-of-sars-cov-2-replication-organelles) [![](https://blog.delmic.com/hs-fs/hubfs/Blogpost_MotorProteins@2x-1.png?width=380&name=Blogpost_MotorProteins@2x-1.png) ](https://blog.delmic.com/cn-blog/cryo-electron-tomography-imaging-flagellar-motor-protein-structures) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [冷冻电子断层扫描：鞭毛运动蛋白结构成像](https://blog.delmic.com/cn-blog/cryo-electron-tomography-imaging-flagellar-motor-protein-structures) ## 推荐阅读 - ## 1 [冷冻电子断层扫描：鞭毛运动蛋白结构成像](https://blog.delmic.com/cn-blog/cryo-electron-tomography-imaging-flagellar-motor-protein-structures) - ## 2 [体电子显微镜：揭示生物样本中的相互作用网](https://blog.delmic.com/cn-blog/electron-volume-microscopy-to-unveil-interaction-networks-in-biological-specimens) - ## 3 [时间分辨阴极发光成像如何拓宽（复合）半导体在新技术中的应用？](https://blog.delmic.com/cn-blog/three-advantages-of-sparc-2) - ## 4 [冷冻电镜 | cryo-ET工作流程中的冰污染问题及其解决方法](https://blog.delmic.com/cn-blog/preventing-ice-contamination-in-the-cryo-et-workflow) - ## 5 [相干和非相干阴极发光有何区别？](https://blog.delmic.com/cn-blog/three-advantages-of-sparc-0) [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20201021meteorblog.png?width=380&name=20201021meteorblog.png) ](https://blog.delmic.com/cn-blog/meteor-first-integrated-cryo-clem-system) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [METEOR: 首个一体化的冷冻光电关联成像系统](https://blog.delmic.com/cn-blog/meteor-first-integrated-cryo-clem-system) 在我们之前的博文中，我们讨论了冷冻电子断层扫描(cryo-ET)的不同应用，以及一体化荧光显微镜(FLM)如何使这项研究受益。在这篇博文中，我们将深入了解Delmic全新的一体化荧光显微镜模块系统METEOR，并阐释它如何帮助简化您的研究。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200529-cryo-blog.png?width=380&name=20200529-cryo-blog.png) ](https://blog.delmic.com/cn-blog/how-can-an-optimized-cryo-et-workflow-benefit-the-research-of-viral-infection) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [优化的冷冻电子断层扫描工作流程如何有益于病毒感染的研究？](https://blog.delmic.com/cn-blog/how-can-an-optimized-cryo-et-workflow-benefit-the-research-of-viral-infection) 病毒是无法在其宿主外繁殖的微观传染原。病毒与多种疾病有直接联系，从常见的病毒（比如，流感病毒或鼻病毒）到当前爆发的新冠病毒[1]。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200616_1_Cryo%20Blogpost%20Epithelial%20Cell-01.png?width=380&name=20200616_1_Cryo%20Blogpost%20Epithelial%20Cell-01.png) ](https://blog.delmic.com/cn-blog/how-can-an-optimized-cryo-et-workflow-benefit-membrane-trafficking-research) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [优化的冷冻电子断层扫描工作流程如何使跨膜运输研究受益？](https://blog.delmic.com/cn-blog/how-can-an-optimized-cryo-et-workflow-benefit-membrane-trafficking-research) 真核细胞包含一个复杂的膜结合细胞器网络，作为细胞活动所需过程的场所。 ### 通过邮件接收最新动态 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200429_cryoFM_blogpost-01.jpg?width=380&name=20200429_cryoFM_blogpost-01.jpg) ](https://blog.delmic.com/cn-blog/limitations-and-possibilities-of-cryogenic-fluorescent-light-microscopy-cryo-flm) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [冷冻荧光显微镜的局限性和可能性](https://blog.delmic.com/cn-blog/limitations-and-possibilities-of-cryogenic-fluorescent-light-microscopy-cryo-flm) 冷冻电子显微镜（cryo-EM）中将样本通过快速冷冻被固定的过程称为玻璃化。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200331%20Cryo%20Blog%20Post.png?width=380&name=20200331%20Cryo%20Blog%20Post.png) ](https://blog.delmic.com/cn-blog/using-integrated-fluorescence-light-microscopy-to-improve-the-cryo-electron-tomography-workflow) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [使用集成的荧光显微镜来改善冷冻电子断层扫描的工作流程](https://blog.delmic.com/cn-blog/using-integrated-fluorescence-light-microscopy-to-improve-the-cryo-electron-tomography-workflow)