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Last year we started to host our podcast-like webinar series, the Delmic Cryo-Electron Tomography (Cryo-ET) Journal Club. In this way we were hoping to share the main ideas of some interesting newly-published papers in a short time. Our sessions could be joined live to participate in the discussion with our hosts. We’ve proudly hosted three episodes in 2020 and there will be more fresh topics covering various applications this year!
Recap of 2020 journal club episodes
- Episode 1: In the first episode our hosts, Marit Smeets and Dr. Caspar Jonker, reviewed the article Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells. By taking excellent use of the diverse capabilities of the cryo-ET technique, the authors identified a new membranous compartment and perform sub-tomogram averaging to get structural information on protein complexes.
- Episode 2: The application of in-situ cryo-ET in coronavirus research. The year 2020 was all about coronavirus and the whole research community was interested in resolving the structure of SARS-CoV-2. Therefore, we discussed two preprints about the structure and replication of coronaviruses are characterized using in situ cryo-ET in combination with FIB milling. 1) A molecular pore spans the double membrane of the coronavirus replication organelle 2) SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography
- Episode 3: Using cryo-ET to understand Parkinson's disease. In this episode our hosts discussed the article The In Situ Structure of Parkinson’s Disease-Linked LRRK2 that resolved LRRK2 using correlative light and electron microscopy, in situ cryo-electron tomography, and subtomogram analysis.
If you missed out these episodes or you want to recap the discussions we had, sign up the series to receive all the recordings in your email! By doing this, we will also make sure to keep you posted for the upcoming topics in 2021!
This work is supported by the European SME2 grant № 879673 - Cryo-SECOM Workflow.
