In the article, they share their opinion on the potential of three-dimensional microscopy for imaging behaviour and development in biological systems.
The review starts with the most recent developments in volume imaging in both light and electron microscopy, and then focuses on the status, potential, and challenges of three-dimensional correlative microscopy. Ultimately, the aim is to understand how behavioural or functional characteristics that can be visualized by light microscopy are related to the biological structure or architecture of entire organs such as the brain.
The full article can be read here.