We’re excited to unveil METEOR 2.0, the next evolution of our integrated cryo-fluorescence microscope (cryo-FM) designed to simplify and accelerate your cryo-electron tomography (cryo-ET) workflow.

Building on the proven foundation of METEOR 1.0, the new system offers faster targeting, greater sensitivity, and enhanced ease of use, allowing researchers to confidently identify and target regions of interest—without added contamination risk or workflow delays.

Building on a strong foundation

METEOR  2.0 builds upon the foundation laid by its predecessor, METEOR 1.0

1. Integrated Fluorescence Microscope: The integration of a  fluorescence microscope within the cryo-FIB/SEM system shortens the workflow and reduces the risk of sample damage and contamination. 

2. Enhanced Targeting Accuracy Equipped with high numerical aperture (NA) objectives, METEOR provides high-resolution images that improve targeting accuracy. 

3. Sensitive Detection: The system includes sensitive cameras and high-NA objectives to maximize light collection and minimize background noise. This ensures that even faint signals are detected, allowing for confirmation of the region of interest in the final lamella. 

4. A Well Loved System With over 25 customers in the field and over 10 publications, METEOR is a trustworthy partner for any cryo-ET lab. 

What's new in METEOR 2.0?

Expanded Field of View
METEOR 2.0  features an 8× larger field of view, enabling faster sample screening and ROI identification. Additionally, capturing more fiducials in a single image enhances 3D correlation and improves targeting accuracy. The expanded context within each frame is especially valuable for large, high-pressure, or waffle-frozen specimens—helping you navigate complex samples with greater confidence and precision.

Advanced Filter Wheel
The new high-speed, high-capacity filter wheel supports up to six customizable single band-pass emission filters, along with reflection imaging. It minimizes spectral bleed-through for cleaner multi-channel imaging and more accurate signal interpretation. Faster switching between channels also boosts imaging throughput, making your workflow more efficient.

Optimized Optical Path
METEOR 2.0’s re-engineered optical path is designed for maximum photon collection and minimal signal loss. Combined with high-NA objectives and high-QE cameras, it enables the reliable detection of even the weakest fluorescence signals using minimal excitation power. This minimizes devitrification risks and gives you full confidence that your region of interest is captured in the final lamella.

Software integration
The ODEMIS software, integral to the METEOR system, facilitates seamless operation by integrating imaging, correlation, and automation functionalities. It supports easy data import and compatibility with external software, enhancing workflow efficiency.

Workflow Enhancements

METEOR 2.0's improvements translate into tangible benefits for researchers:

• Reduced Contamination: By imaging directly in the cryo-FIB/SEM chamber, the system minimizes contamination.
• Time Efficiency: The combination of a large field of view, a fast filter wheel, and smart software functionalities accelerates sample screening and imaging processes.
• Improved Image Quality: High numerical aperture objectives, high quantum efficiency cameras, and an optimized optical path ensure high-resolution images even at low illumination powers.
• Reduced user interaction: The powerful software allows users to step away from the microscope and attend to their actual research (or finally answer that one email). 

Conclusion

METEOR 2.0 is designed to enable researchers to "see more with less" by providing a system that reduces contamination, saves time, and enhances imaging capabilities. Its advanced features and software integration make it a powerful tool for the growing demands of cryo-ET. With METEOR 2.0, researchers can achieve more accurate and efficient results, paving the way for groundbreaking discoveries in the field.