cryo electron microscopy
Subtomogram averaging in the cryo-ET workflow
Cryo-electron tomography (cryo-ET) allows the 3D visualization of a small volume of a vitreous biological sample with nanometer resolution. Protein complexes that reside in the tomogram can be studied in even greater detail with subtomogram averaging. Subtomogram averaging is the process of aligning multiple 3D reconstructions of identical protein complexes and averaging them.