--- title: Delmic 博客 description: Delmic 博客 --- ###### [超快体电子显微镜](https://blog.delmic.com/cn-blog/tag/超快体电子显微镜) ##### [电镜制样 | 从层层瓶颈到全流程自动化](https://blog.delmic.com/cn-blog/sample-preparation-in-electron-microscopy-from-bottleneck-to-automated-process) 样品制备在电子显微镜（EM）中是至关重要的，因为它对最终数据的影响与电子显微镜质量造成的影响一样重大，甚至可能更大。由于其耗时性，它往往被视为生物样品的高通量电镜的瓶颈。这篇文章解释了电镜的样品制备几个关键步骤，并给出了几种可能的解决方案，以加快并自动化这个过程。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/METEOR_MillingWorkflow_Blogpost.png?width=380&name=METEOR_MillingWorkflow_Blogpost.png) ](https://blog.delmic.com/cn-blog/focused-ion-beam-sample-preparation-for-cryo-electron-tomography) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [用于冷冻电子断层扫描的聚焦离子束（FIB）样品制备](https://blog.delmic.com/cn-blog/focused-ion-beam-sample-preparation-for-cryo-electron-tomography) 冷冻电子断层扫描（cryo-ET）是一种非常强大的技术，可以在接近原生状态下对细胞结构进行高分辨率的研究。由于厚于600纳米的生物样品不对电子透明，因此需要减薄才能用于冷冻电子断层扫描。为了使样品变薄，使用带有聚焦离子束和冷冻载物台的扫描电子显微镜(cryo-FIB/SEM)已成为黄金标准。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/DELMIC_UVC_LEDS_APPLICATIONNOTE_BLOGBANNER.png?width=380&name=DELMIC_UVC_LEDS_APPLICATIONNOTE_BLOGBANNER.png) ](https://blog.delmic.com/cn-blog/studying-uv-led-materials-with-cathodoluminescence-imaging) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [利用阴极发光成像技术研究紫外发光二极管（UV LED）新型半导体材料](https://blog.delmic.com/cn-blog/studying-uv-led-materials-with-cathodoluminescence-imaging) 阴极发光通常用于探测半导体发光二极管（LED）材料的光发射特性。 [![](https://blog.delmic.com/hs-fs/hubfs/DMVPores_SARS-CoV-2.png?width=380&name=DMVPores_SARS-CoV-2.png) ](https://blog.delmic.com/cn-blog/cryo-et-imaging-of-sars-cov-2-replication-organelles) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [冷冻电子断层扫描的应用：成像新冠病毒复制细胞器](https://blog.delmic.com/cn-blog/cryo-et-imaging-of-sars-cov-2-replication-organelles) 2... [![](https://blog.delmic.com/hs-fs/hubfs/Blogpost_MotorProteins@2x-1.png?width=380&name=Blogpost_MotorProteins@2x-1.png) ](https://blog.delmic.com/cn-blog/cryo-electron-tomography-imaging-flagellar-motor-protein-structures) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [冷冻电子断层扫描：鞭毛运动蛋白结构成像](https://blog.delmic.com/cn-blog/cryo-electron-tomography-imaging-flagellar-motor-protein-structures) 鞭毛是在许多细菌和一些真核细胞上发现的丝状蛋白复合物。随着成像技术（如冷冻电子断层扫描）的不断发展，我们能够更好地掌握这些复杂的运动蛋白如何发挥作用和参与感应链反应。在这篇文章中，我们将通过最近的一些研究来探讨成像技术是如何发展的，这些发展将如何助力我们深入了解蛋白质结构，比如鞭毛运动系统。 [![](https://blog.delmic.com/hs-fs/hubfs/FastEM_Blogpost_InvasionMetasisCascade@2x-2.png?width=380&name=FastEM_Blogpost_InvasionMetasisCascade@2x-2.png) ](https://blog.delmic.com/cn-blog/when-3d-electron-microscopy-meets-cancer-research) ###### [超快体电子显微镜](https://blog.delmic.com/cn-blog/tag/超快体电子显微镜) ##### [当3D电镜遇上癌症研究](https://blog.delmic.com/cn-blog/when-3d-electron-microscopy-meets-cancer-research) 三维电子显微镜(3D电镜)在细胞生物学和诊断病理学领域的应用越来越多，这是因为它是在纳米尺度上获得整个超细胞结构概况的最准确方法之一。由于癌症是一种表现在细胞水平上的疾病，因此电镜在癌症研究和诊断中的应用越来越重要。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/InGan.png?width=380&name=InGan.png) ](https://blog.delmic.com/cn-blog/three-advantages-of-sparc-2) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [时间分辨阴极发光成像如何拓宽（复合）半导体在新技术中的应用？](https://blog.delmic.com/cn-blog/three-advantages-of-sparc-2) 半导体作为电子设备的重要组成部分，在过去的几十年中一直是材料研究的重点。硅因其易用性而成为最常见的半导体材料，并已发现了广泛的商业应用。然而，硅是一种间接的带隙材料，这在一定程度上限制了其应用。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20201021meteorblog.png?width=380&name=20201021meteorblog.png) ](https://blog.delmic.com/cn-blog/meteor-first-integrated-cryo-clem-system) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [METEOR: 首个一体化的冷冻光电关联成像系统](https://blog.delmic.com/cn-blog/meteor-first-integrated-cryo-clem-system) 在我们之前的博文中，我们讨论了冷冻电子断层扫描(cryo-ET)的不同应用，以及一体化荧光显微镜(FLM)如何使这项研究受益。在这篇博文中，我们将深入了解Delmic全新的一体化荧光显微镜模块系统METEOR，并阐释它如何帮助简化您的研究。 [![](https://blog.delmic.com/hs-fs/hubfs/EMPA%20paper%20CCLEM.gif?width=380&name=EMPA%20paper%20CCLEM.gif) ](https://blog.delmic.com/cn-blog/three-advantages-of-sparc-1) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [最新论文：阴极发光电镜关联技术的生物成像](https://blog.delmic.com/cn-blog/three-advantages-of-sparc-1) 近日，Empa、苏黎世联邦理工学院和Delmic的研究人员共同发表了一篇新文章，作者们探索了阴极发光电镜关联技术（Correlative cathodoluminescence electron microscopy）的使用，以及阴极发光电镜关联技术应用于（体积）多色标记的可能性。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/Fast-em%20blog%20post.png?width=380&name=Fast-em%20blog%20post.png) ](https://blog.delmic.com/cn-blog/from-imaging-to-analysing-how-delmic-new-fast-em-system-is-changing-electron-microscopy) ###### [超快体电子显微镜](https://blog.delmic.com/cn-blog/tag/超快体电子显微镜) ##### [从成像到分析：Delmic的新型FAST-EM系统如何重新定义超快体电子显微镜](https://blog.delmic.com/cn-blog/from-imaging-to-analysing-how-delmic-new-fast-em-system-is-changing-electron-microscopy) 在之前的文章中，我们讨论了更快更高效的电镜系统能够如何加快大型项目的开发速度，使电镜成像中心受益，同时让研究人员专注于分析数据而不是成像过程。今天，我们将重点介绍Delmic的新型多光束系统FAST-EM，并阐释它将如何实现可持续的高通量。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200529-cryo-blog.png?width=380&name=20200529-cryo-blog.png) ](https://blog.delmic.com/cn-blog/how-can-an-optimized-cryo-et-workflow-benefit-the-research-of-viral-infection) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [优化的冷冻电子断层扫描工作流程如何有益于病毒感染的研究？](https://blog.delmic.com/cn-blog/how-can-an-optimized-cryo-et-workflow-benefit-the-research-of-viral-infection) 病毒是无法在其宿主外繁殖的微观传染原。病毒与多种疾病有直接联系，从常见的病毒（比如，流感病毒或鼻病毒）到当前爆发的新冠病毒[1]。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/News/Coherent%20and%20incoherent%20CL.png?width=380&name=Coherent%20and%20incoherent%20CL.png) ](https://blog.delmic.com/cn-blog/three-advantages-of-sparc-0) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [相干和非相干阴极发光有何区别？](https://blog.delmic.com/cn-blog/three-advantages-of-sparc-0) 阴极发光过程可分为两类：相干阴极发光和非相干阴极发光。这两种之间有什么区呢别？在这篇文章中，我们将概述各种类型的阴极发光，并解释阴极发光成像的目的，最后定义相干和非相干阴极发光。 [![](https://blog.delmic.com/hubfs/Website/Delmic%20logos/Product%20icons/SPARC%20Spectral/SPARC_SPECTRAL_Logo_Turquoise.svg) ](https://blog.delmic.com/cn-blog/three-advantages-of-sparc) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [SPARC阴极发光探测器的三大优势](https://blog.delmic.com/cn-blog/three-advantages-of-sparc) SPARC阴极发光探测器的主要优势是什么呢？ [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200722_CL%20for%20geological%20materials.png?width=380&name=20200722_CL%20for%20geological%20materials.png) ](https://blog.delmic.com/cn-blog/observing-defects-in-rocks-with-cathodoluminescence) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [使用阴极发光观察岩石中的缺陷](https://blog.delmic.com/cn-blog/observing-defects-in-rocks-with-cathodoluminescence) 阴极发光通常用于预筛选地质样本，并与其他扫描电镜技术结合使用以进行深入研究。阴极发光成像的多种模式可以提供许多有关岩石结构的信息，其中包括岩石的缺陷。那么在晶体中可以观察到哪些类型的缺陷，以及如何检测到它们呢？ [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200615%20Blog%20post%20Fast%20EM.png?width=380&name=20200615%20Blog%20post%20Fast%20EM.png) ](https://blog.delmic.com/cn-blog/how-can-fast-electron-microscopy-maintain-both-context-and-high-resolution-in-large-projects) ###### [超快体电子显微镜](https://blog.delmic.com/cn-blog/tag/超快体电子显微镜) ##### [超快体电镜如何在大型成像项目中维持高分辨率且洞悉细胞环境](https://blog.delmic.com/cn-blog/how-can-fast-electron-microscopy-maintain-both-context-and-high-resolution-in-large-projects) 在现代医学研究中，比较健康和患病的细胞或组织的形态或检查药物治疗的效果对于理解潜在的生命机制极为重要。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200616_1_Cryo%20Blogpost%20Epithelial%20Cell-01.png?width=380&name=20200616_1_Cryo%20Blogpost%20Epithelial%20Cell-01.png) ](https://blog.delmic.com/cn-blog/how-can-an-optimized-cryo-et-workflow-benefit-membrane-trafficking-research) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [优化的冷冻电子断层扫描工作流程如何使跨膜运输研究受益？](https://blog.delmic.com/cn-blog/how-can-an-optimized-cryo-et-workflow-benefit-membrane-trafficking-research) 真核细胞包含一个复杂的膜结合细胞器网络，作为细胞活动所需过程的场所。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200429_cryoFM_blogpost-01.jpg?width=380&name=20200429_cryoFM_blogpost-01.jpg) ](https://blog.delmic.com/cn-blog/limitations-and-possibilities-of-cryogenic-fluorescent-light-microscopy-cryo-flm) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [冷冻荧光显微镜的局限性和可能性](https://blog.delmic.com/cn-blog/limitations-and-possibilities-of-cryogenic-fluorescent-light-microscopy-cryo-flm) 冷冻电子显微镜（cryo-EM）中将样本通过快速冷冻被固定的过程称为玻璃化。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/Fast%20Imaging.png?width=380&name=Fast%20Imaging.png) ](https://blog.delmic.com/cn-blog/how-does-an-imaging-facility-benefit-from-fast-imaging) ###### [超快体电子显微镜](https://blog.delmic.com/cn-blog/tag/超快体电子显微镜) ##### [成像中心如何从超快体电镜成像中受益？](https://blog.delmic.com/cn-blog/how-does-an-imaging-facility-benefit-from-fast-imaging) 同时管理大量的研究项目已经成为了现如今的成像中心们所面临的一大困难。 [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200513_Illustration_Sonic_Workflow-01.png?width=380&name=20200513_Illustration_Sonic_Workflow-01.png) ](https://blog.delmic.com/cn-blog/electron-volume-microscopy-to-unveil-interaction-networks-in-biological-specimens) ###### [超快体电子显微镜](https://blog.delmic.com/cn-blog/tag/超快体电子显微镜) ##### [体电子显微镜：揭示生物样本中的相互作用网](https://blog.delmic.com/cn-blog/electron-volume-microscopy-to-unveil-interaction-networks-in-biological-specimens) [![](https://blog.delmic.com/hs-fs/hubfs/Website%20Content%20Offers/Webinar%20thumbnails%20full%20size/Thumbnail%20Webinar%20Rare-earth%20materials%20cathodoluminescence%20full.png?width=380&name=Thumbnail%20Webinar%20Rare-earth%20materials%20cathodoluminescence%20full.png) ](https://blog.delmic.com/cn-blog/cl-for-rare-earth-doped-materials-webinar-review) ###### [阴极发光](https://blog.delmic.com/cn-blog/tag/阴极发光) ##### [经典网络研讨会回顾 | 如何用阴极发光观察稀土掺杂材料](https://blog.delmic.com/cn-blog/cl-for-rare-earth-doped-materials-webinar-review) [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200331%20Cryo%20Blog%20Post.png?width=380&name=20200331%20Cryo%20Blog%20Post.png) ](https://blog.delmic.com/cn-blog/using-integrated-fluorescence-light-microscopy-to-improve-the-cryo-electron-tomography-workflow) ###### [cryo-electron tomography](https://blog.delmic.com/cn-blog/tag/cryo-electron-tomography) ##### [使用集成的荧光显微镜来改善冷冻电子断层扫描的工作流程](https://blog.delmic.com/cn-blog/using-integrated-fluorescence-light-microscopy-to-improve-the-cryo-electron-tomography-workflow) [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200213_Fast-Imaging%20blogpost-01.png?width=380&name=20200213_Fast-Imaging%20blogpost-01.png) ](https://blog.delmic.com/cn-blog/optimizing-for-high-sustained-throughput-in-large-scale-electron-microscopy) ###### [fast SEM imaging](https://blog.delmic.com/cn-blog/tag/fast-sem-imaging) ##### [工作流程优化：实现高通量可持续大规模电子显微成像](https://blog.delmic.com/cn-blog/optimizing-for-high-sustained-throughput-in-large-scale-electron-microscopy) [![](https://blog.delmic.com/hs-fs/hubfs/image-2.png?width=380&name=image-2.png) ](https://blog.delmic.com/cn-blog/how-can-time-resolved-cathodoluminescence-imaging-expand-applications-of-compound-semiconductors-in-new-technologies) ###### [阴极发光](https://blog.delmic.com/cn-blog/tag/阴极发光) ##### [时间分辨阴极发光成像如何拓宽（复合）半导体在新技术中的应用？](https://blog.delmic.com/cn-blog/how-can-time-resolved-cathodoluminescence-imaging-expand-applications-of-compound-semiconductors-in-new-technologies) [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200213_Fast-Imaging%20blogpost-01.png?width=380&name=20200213_Fast-Imaging%20blogpost-01.png) ](https://blog.delmic.com/cn-blog/overcoming-the-challenges-of-large-scale-electron-microscopy) ###### [fast SEM imaging](https://blog.delmic.com/cn-blog/tag/fast-sem-imaging) ##### [自动超快体扫描电镜：克服大规模成像的种种挑战](https://blog.delmic.com/cn-blog/overcoming-the-challenges-of-large-scale-electron-microscopy) [![](https://blog.delmic.com/hs-fs/hubfs/Webinar%20time%20resolved%20cathodoluminescence%20(CL).png?width=380&name=Webinar%20time%20resolved%20cathodoluminescence%20(CL).png) ](https://blog.delmic.com/cn-blog/how-to-perform-lifetime-imaging-using-time-resolved-cathodoluminescence) ###### [time-resolved cathodoluminescence](https://blog.delmic.com/cn-blog/tag/time-resolved-cathodoluminescence) ##### [如何使用时间分辨阴极发光进行光子寿命探测？](https://blog.delmic.com/cn-blog/how-to-perform-lifetime-imaging-using-time-resolved-cathodoluminescence) [![](https://blog.delmic.com/hs-fs/hubfs/JOLT%20banner.png?width=380&name=JOLT%20banner.png) ](https://blog.delmic.com/cn-blog/jolt-intensity-detector) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [JOLT：刚刚好是你需要的阴极发光强度探测器](https://blog.delmic.com/cn-blog/jolt-intensity-detector) [![](https://blog.delmic.com/hs-fs/hubfs/perovskite%20cathodoluminescence%20intensity%20map.png?width=380&name=perovskite%20cathodoluminescence%20intensity%20map.png) ](https://blog.delmic.com/cn-blog/review-on-cl-halide-perovskites) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [学术前沿 | 关于卤化物钙钛矿阴极发光分析的最新评论](https://blog.delmic.com/cn-blog/review-on-cl-halide-perovskites) [![](https://blog.delmic.com/hs-fs/hubfs/Website%20Content%20Offers/Webinar%20thumbnails%20full%20size/Thumbnail%20Webinar%20Imaging%20conditions%20for%20CL%20full.png?width=380&name=Thumbnail%20Webinar%20Imaging%20conditions%20for%20CL%20full.png) ](https://blog.delmic.com/cn-blog/what-are-the-optimal-imaging-conditions-for-cathodoluminescence) ###### [cathodoluminescence](https://blog.delmic.com/cn-blog/tag/cathodoluminescence) ##### [如何设置阴极发光的最佳成像条件？](https://blog.delmic.com/cn-blog/what-are-the-optimal-imaging-conditions-for-cathodoluminescence) [![](https://blog.delmic.com/hs-fs/hubfs/Website%20Content%20Offers/CLEM%20Application%20Notes/Thumbnails%20CLEM%20application%20notes/Image%20Thumbnail%20Application%20note%20CLEM%20Type%201%20DiabetesRES_270x153.jpg?width=380&name=Image%20Thumbnail%20Application%20note%20CLEM%20Type%201%20DiabetesRES_270x153.jpg) ](https://blog.delmic.com/cn-blog/how-to-image-beta-cells-in-the-islets-of-langerhans-fast-and-clearly) ###### 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