--- title: Delmic Microscopy Blog | cryo-electron tomography (2) description: cryo-electron tomography | This blog addresses the various applications and techniques to be discovered in the fields of cathodoluminescence and correlative light and electron microscopy. (2) --- [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/2022_Cryo_Webinar_CERES%20IS%20Ice%20Growth_Website.png?width=380&name=2022_Cryo_Webinar_CERES%20IS%20Ice%20Growth_Website.png) ](https://blog.delmic.com/protecting-lamellae-from-ice-contamination-especially-during-the-fine-polishing-step-in-the-cryo-et-workflow) ###### [life sciences](https://blog.delmic.com/topic/life-sciences) ##### [Protecting lamellae from ice contamination during milling in the cryo-ET workflow](https://blog.delmic.com/protecting-lamellae-from-ice-contamination-especially-during-the-fine-polishing-step-in-the-cryo-et-workflow) Sample milling is a crucial step in the Cryo-ET workflow. Unfortunately, this step poses yet another occasion for ice contamination to derail efforts to obtain ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/Cryo_Muscle%20Proteins%203D%20Tomography_Website.png?width=380&name=Cryo_Muscle%20Proteins%203D%20Tomography_Website.png) ](https://blog.delmic.com/uncovering-3d-molecular-organization-and-interactions-between-muscle-proteins-using-in-situ-cryo-electron-tomography) ###### [life sciences](https://blog.delmic.com/topic/life-sciences) ##### [Uncovering 3D molecular organization and interactions between muscle proteins using in situ cryo-electron tomography](https://blog.delmic.com/uncovering-3d-molecular-organization-and-interactions-between-muscle-proteins-using-in-situ-cryo-electron-tomography) There are more than 150 known musculoskeletal conditions (1), many of which affect specifically skeletal muscles and can lead to lifelong disabilities or even ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/CERES_Clean%20Station_Blogpost_Website.png?width=380&name=CERES_Clean%20Station_Blogpost_Website.png) ](https://blog.delmic.com/how-to-keep-the-cryo-em-sample-clean-and-safe-during-sample-preparation-process) ###### [life sciences](https://blog.delmic.com/topic/life-sciences) ##### [How to keep the cryo-EM sample clean and safe during sample preparation process?](https://blog.delmic.com/how-to-keep-the-cryo-em-sample-clean-and-safe-during-sample-preparation-process) Cryo-EM research helps us understand how the world works on a microscale. Often these insights have a great impact on our lives, for example, the understanding ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/CERES_Vitri%20Lock%20Blogpost_Website.png?width=380&name=CERES_Vitri%20Lock%20Blogpost_Website.png) ](https://blog.delmic.com/how-to-minimize-ice-formation-during-sample-transfer-in-cryo-et) ###### [life sciences](https://blog.delmic.com/topic/life-sciences) ##### [How to minimize ice formation during sample transfer in cryo-ET?](https://blog.delmic.com/how-to-minimize-ice-formation-during-sample-transfer-in-cryo-et) Sample transfer is definitely not the most spectacular part of the cryo-ET workflow. And yet, it is one of the most problematic steps, disturbing many ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/Sample%20Prep%20Cryo-ET_Website.png?width=380&name=Sample%20Prep%20Cryo-ET_Website.png) ](https://blog.delmic.com/how-to-streamline-the-cryo-et-workflow-and-get-more-useful-biological-insights) ###### [life sciences](https://blog.delmic.com/topic/life-sciences) ##### [How to streamline the cryo-ET workflow and get more useful biological insights](https://blog.delmic.com/how-to-streamline-the-cryo-et-workflow-and-get-more-useful-biological-insights) Cryo-electron tomography (cryo-ET) is an extremely powerful technique that allows studies of the cellular landscape at high resolution in a near-native state. [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/2021_HeLA%20Yeast%20Blogpost_Website%20(1).png?width=380&name=2021_HeLA%20Yeast%20Blogpost_Website%20(1).png) ](https://blog.delmic.com/applications-of-an-integrated-cryogenic-fluorescent-light-microscope) ###### [life sciences](https://blog.delmic.com/topic/life-sciences) ##### [Applications of an integrated cryogenic fluorescent light microscope](https://blog.delmic.com/applications-of-an-integrated-cryogenic-fluorescent-light-microscope) Do you know how an integrated cryogenic fluorescent light microscope can streamline the cryo-ET workflow for targeted lamella milling of HeLa and yeast cells? ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/CRYO_Ice%20Contamination_Website_Labeled.png?width=380&name=CRYO_Ice%20Contamination_Website_Labeled.png) ](https://blog.delmic.com/preventing-ice-contamination-in-single-particle-cryo-electron-microscopy) ###### [life sciences](https://blog.delmic.com/topic/life-sciences) ##### [Preventing ice contamination in single-particle electron microscopy](https://blog.delmic.com/preventing-ice-contamination-in-single-particle-cryo-electron-microscopy) There is an increasing number of institutes that are starting to employ cryo-EM. However, many challenges are involved and specimen preparation is still a ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20210420_AprilBlog_Preventing%20Ice%20Contamination.jpg?width=380&name=20210420_AprilBlog_Preventing%20Ice%20Contamination.jpg) ](https://blog.delmic.com/preventing-ice-contamination-in-the-cryo-et-workflow) ###### [life sciences](https://blog.delmic.com/topic/life-sciences) ##### [Preventing ice contamination in the cryo-ET workflow](https://blog.delmic.com/preventing-ice-contamination-in-the-cryo-et-workflow) One of the biggest hurdles in the current cryo-ET workflow is avoiding ice contamination of the vitreous sample, which is difficult to avoid due to the many ... [![](https://blog.delmic.com/hs-fs/hubfs/METEOR_GuidedCryoLamellae_BlogHeader.png?width=380&name=METEOR_GuidedCryoLamellae_BlogHeader.png) ](https://blog.delmic.com/preparing-cryo-lamellae-with-the-guidance-of-meteor) ###### [cryo electron microscopy](https://blog.delmic.com/topic/cryo-electron-microscopy) ##### [Preparing cryo-lamellae with the guidance of METEOR](https://blog.delmic.com/preparing-cryo-lamellae-with-the-guidance-of-meteor) METEOR, our integrated top down fluorescence light microscope (FLM) increases lamella sample preparation for cryo-electron tomography imaging (cryo-ET). This ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20201021meteorblog.png?width=380&name=20201021meteorblog.png) ](https://blog.delmic.com/meteor-an-integrated-top-down-cryo-clem-imaging-system) ###### [cryo electron microscopy](https://blog.delmic.com/topic/cryo-electron-microscopy) ##### [METEOR: an integrated top down cryo-CLEM imaging system](https://blog.delmic.com/meteor-an-integrated-top-down-cryo-clem-imaging-system) In our previous blogposts we discussed different applications of cryogenic electron tomography (cryo-ET) and how an integrated fluorescent light microscope ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/METEOR_Banner_SubtomographyAveraging_Collection.png?width=380&name=METEOR_Banner_SubtomographyAveraging_Collection.png) ](https://blog.delmic.com/subtomogram-averaging-in-the-cryo-et-workflow) ###### [cryo electron microscopy](https://blog.delmic.com/topic/cryo-electron-microscopy) ##### [Subtomogram averaging in the cryo-ET workflow](https://blog.delmic.com/subtomogram-averaging-in-the-cryo-et-workflow) Cryo-electron tomography (cryo-ET) allows the 3D visualization of a small volume of a vitreous biological sample with nanometer resolution. Protein complexes ... [![](https://blog.delmic.com/hs-fs/hubfs/Website/Blog/20200806%20cryo%20blog%20august.png?width=380&name=20200806%20cryo%20blog%20august.png) ](https://blog.delmic.com/recent-advances-in-automated-lamellae-milling) ###### [cryo electron microscopy](https://blog.delmic.com/topic/cryo-electron-microscopy) ##### [Recent advances in automated lamellae milling](https://blog.delmic.com/recent-advances-in-automated-lamellae-milling) Cryo-electron tomography (cryo-ET) is an emerging technique that allows scientists to determine the structure of biological complexes within their cellular ... - [1](https://blog.delmic.com/topic/cryo-electron-tomography) - [2](https://blog.delmic.com/topic/cryo-electron-tomography/page/2) - [3](https://blog.delmic.com/topic/cryo-electron-tomography/page/3) - [![→](https://blog.delmic.com/hubfs/system/icons/arrow-right-black.svg)](https://blog.delmic.com/topic/cryo-electron-tomography/page/3)